Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Molecular Devices Fluorescence Polarization Filter, 360 nm, 35 nm, Horizontal
Fluorescence polarization filter slide with integrated holder for microplate readers. Optimized for excitation at 360 nm with a 35 nm optical bandwidth and horizontal polarization orientation, this filter is used in fluorescence polarization assays to measure molecular binding and rotational mobility.
- Designed for fluorescence polarization measurements
- Optimized excitation wavelength 360 nm with 35 nm bandwidth
- Horizontal polarization orientation for emission analysis
- Includes an integrated holder for secure installation in filter slides
- Compatible with multi-mode microplate readers that accept filter slides
Revvity Health Sciences Inc HTRF Human Phospho-Cyclin D1 (Thr286) Detection Kit, 500 Assay Points
HTRF Human Phospho-Cyclin D1 (Thr286) Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Amyloid β 1-42 Detection Kit, 10,000 Assay Points
HTRF Human Amyloid β 1-42 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-ATM (Ser1981) Detection Kit, 50,000 Assay Points
HTRF Human Phospho-ATM (Ser1981) Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human XIAP / BIR3 Binding Kit, 10,000 Assay Points
HTRF Human XIAP / BIR3 Binding Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total p300 Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Total p300 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-JAK1 (Tyr1034/1035) Detection Kit, 500 Assay Points
HTRF Human Phospho-JAK1 (Tyr1034/1035) Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF High Performance Human IL-6 Detection Kit, 100,000 Assay Points
HTRF High Performance Human IL-6 Detection Kit, 100,000 Assay Points
Revvity Health Sciences Inc HTRF Active GLP1 Detection Kit, 10,000 Assay Points
HTRF Active GLP1 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total H2AX Detection Kit, Control Lysate
HTRF Human Total H2AX Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF cIAP1 BIR3 Binding Kit, 100,000 Assay Points
HTRF cIAP1 BIR3 Binding Kit, 100,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total ATG14 Detection Kit, 50,000 Assay Points
HTRF Human Total ATG14 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human XIAP / BIR2 Binding Kit, 500 Assay Points
HTRF Human XIAP / BIR2 Binding Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Total ULK1 Detection Kit, Control Lysate
HTRF Human Total ULK1 Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF AAV3B Detection Kit, 10,000 Assay Points
HTRF AAV3B Detection Kit, 10,000 Assay Points